Specimen Stability (for laboratory use only)
Lavender%20top%20(K2-EDTA).png
Collection Container
Lavender top (K2-EDTA)
Specimen Type:
Bone marrow aspirate
Minimum Specimen Volume:
2 mL
Specimen Type:
Blood
Minimum Specimen Volume:
3 mL (6 mL if WBC <5x10⁹/L)
Specimen Type:
Lymph node / solid tumour
Minimum Specimen Volume:
5mm³ or larger biopsy
Specimen Type:
Body fluid
Minimum Specimen Volume:
3 - 6 mL
Collection Instructions:
**Refer to FCM Flow Chart for ordering guide**
https://nuhsingapore.testcatalog.org/catalogs/394/files/6372

Submit one of the following: Bone marrow, peripheral blood, lymph node, solid tumour OR body fluid

For bone marrow collection:
1. Collect bone marrow into lavender top tube.
2. Gently invert the tube 8 - 10 times.
3. Send specimen to the laboratory immediately.

For peripheral blood collection:
1. Draw sufficient blood into lavender top tube.
2. Draw 2 tubes if WBC <5x10⁹/L.
3. Gently invert the tube 8 - 10 times.
4. Send specimen to the laboratory immediately.

For lymph node, solid tumour collection:
1. Prearrange with laboratory for transport medium.
2. Collect specimen directly into 5 mL RPMI 1640 medium after biopsy.
3. Do not leave tissue to air-dry or fix tissue in formalin.
4. Send specimen to the laboratory immediately.

For body fluid collection:
1. Prearrange with laboratory for transport medium
2. Collect specimen into a container with 0.6 mL transfix medium.
3. Send specimen to the laboratory immediately.

All specimens should be sent in ambient temperature (18 - 25˚C). Specimens should be sent to the testing laboratory within 24 - 48 hours of collection. Specimen received after 48 hours of collection will nonetheless be processed but results may be compromised.

Refer to testing laboratory test catalogue for more information:
https://nuhsingapore.testcatalog.org/show/8AML
Unacceptable Conditions:
Frozen
Air-dry or formalin fixed tissues.
Specimen Stability:
Ambient
Send specimen to the laboratory immediately


Refrigerate
Frozen
Performance
Turn Around Time:
5 - 20 days
Days Test Performed:
Testing Laboratory:
National University Hospital (NUH), Flow Cytometry
Clinical & Interpretative
Description:
Methodology:
Flow cytometry
Reference Interval:
See laboratory report
Critical Values:
N.A.