Specimen Stability (for laboratory use only)
Collection Container
Service Code:
LBC00863
Specimen Type:
TBC
Minimum Specimen Volume:
-
Specimen Type:
Minimum Specimen Volume:
1 swab
Specimen Type:
Minimum Specimen Volume:
1mL
Collection Instructions:
Submit one of the following: Nasal, throat, oral, nasopharyngeal washes, sinus flush, bronchoalveolar lavage, bronchial wash, sputum, induced sputum, endotracheal aspirate OR protected specimen brush.

For upper respiratory sites collection:
1. Use the one swab for both sides of the nose.

For throat/oral collection:
1. Carefully swab the site only. Avoid touching the surrounding area.

For bronchoalveolar lavage collection:
1. Please indicate if extended incubation is required when slow-growing bacteria such as Nocardia or Actinomyces are suspected.
2. Collect specimen into a sterile screw capped container.

For expectorated sputum collection:
1. Collect specimen into a sterile screw capped container, under direct supervision of nurse or physician.
2. Have patient rinse mouth with water.
3. Instruct patient to take a very deep breath to fill the lungs, and empty them in one breath, coughing as hard as possible to produce lower respiratory specimen (not saliva or postnasal fluid).

For induced sputum collection:
1. Have patient rinse mouth with water after brushing gums and tongue.
2. Using a nebuliser, have patient inhale ~25 mL of 3-10% sterile saline.
3. Collect induced sputum into sterile screw capped container.

Note: Culture will be rejected if sputum specimen is of poor quality.

Send specimen to the laboratory immediately.
Unacceptable Conditions:
Gastric aspirates
Nasopharyngeal aspirates
Specimen Stability:
Ambient
2 hours for eSwabs
4 hours for all other specimens
Refrigerate
Frozen
Reflex Tests:
N.A.
Performance
Turn Around Time:
3 days
Days Test Performed:
Testing Laboratory:
Woodlands Hospital Laboratory Medicine and Pathology, Medical Microbiology
Clinical & Interpretative
Description:
Methodology:

Upper respiratory sites
Conventional culture

Lower respiratory sites
Conventional culture with direct Gram stain of specimen that are non-swab based.
Reference Interval:

Upper respiratory sites
Normal oral flora isolated

Lower respiratory sites
Respiratory flora isolated
Critical Values:
N.A.