Specimen Stability (for laboratory use only)
Collection Container
Lavender top (K2-EDTA)
Service Code:
LBC01189
Collection Instructions:
**Refer to FCM Flow Chart for ordering guide**
https://nuhsingapore.testcatalog.org/catalogs/394/files/6372
Submit one of the following: Bone marrow, peripheral blood, lymph node, solid tumour OR body fluid
For bone marrow collection:
1. Collect bone marrow into lavender top tube.
2. Gently invert the tube 8 - 10 times.
3. Send specimen to the laboratory immediately.
For peripheral blood collection:
1. Draw sufficient blood into lavender top tube.
2. Draw 2 tubes if WBC <5x10⁹/L.
3. Gently invert the tube 8 - 10 times.
4. Send specimen to the laboratory immediately.
For lymph node, solid tumour collection:
1. Prearrange with laboratory for transport medium.
2. Collect specimen directly into 5 mL RPMI 1640 medium after biopsy.
3. Do not leave tissue to air-dry or fix tissue in formalin.
4. Send specimen to the laboratory immediately.
For body fluid collection:
1. Prearrange with laboratory for transport medium
2. Collect specimen into a container with 0.6 mL transfix medium.
3. Send specimen to the laboratory immediately.
All specimens should be shipped to the lab in ambient temperature (18-25˚C) within 24-48 hours of collection. Specimen received after 48 hours of collection will nonetheless be processed but results may be compromised. For overseas specimens, avoid shipping on Friday or eve of public holidays.
Refer to testing laboratory test catalogue for more information:
https://nuhsingapore.testcatalog.org/show/8TALL
https://nuhsingapore.testcatalog.org/catalogs/394/files/6372
Submit one of the following: Bone marrow, peripheral blood, lymph node, solid tumour OR body fluid
For bone marrow collection:
1. Collect bone marrow into lavender top tube.
2. Gently invert the tube 8 - 10 times.
3. Send specimen to the laboratory immediately.
For peripheral blood collection:
1. Draw sufficient blood into lavender top tube.
2. Draw 2 tubes if WBC <5x10⁹/L.
3. Gently invert the tube 8 - 10 times.
4. Send specimen to the laboratory immediately.
For lymph node, solid tumour collection:
1. Prearrange with laboratory for transport medium.
2. Collect specimen directly into 5 mL RPMI 1640 medium after biopsy.
3. Do not leave tissue to air-dry or fix tissue in formalin.
4. Send specimen to the laboratory immediately.
For body fluid collection:
1. Prearrange with laboratory for transport medium
2. Collect specimen into a container with 0.6 mL transfix medium.
3. Send specimen to the laboratory immediately.
All specimens should be shipped to the lab in ambient temperature (18-25˚C) within 24-48 hours of collection. Specimen received after 48 hours of collection will nonetheless be processed but results may be compromised. For overseas specimens, avoid shipping on Friday or eve of public holidays.
Refer to testing laboratory test catalogue for more information:
https://nuhsingapore.testcatalog.org/show/8TALL
Unacceptable Conditions:
Frozen
Air-dry or formalin fixed tissues.
Air-dry or formalin fixed tissues.
Specimen Stability:
Ambient
Send specimen to the laboratory immediately
Refrigerate
Frozen
Performance
Turn Around Time:
5 - 20 days
Days Test Performed:
Testing Laboratory:
National University Hospital (NUH), Flow Cytometry
Clinical & Interpretative
Description:
Methodology:
Flow cytometry
Reference Interval:
See laboratory report
Critical Values:
N.A.

